Fig 1: (a) Current response (%) for each prepared PS/MWCNT-PAMAM/Anti-CD36 immunosensor and (b) stability study. The PS/MWCNT-PAMAM/Anti-CD36 immunosensors were tested for 5 days of storage at 4 °C (all measurements, scan rate: 50 mVs–1, in the presence of 10 mL of PBS (pH 7.4) solution containing 5 mM HCF and 0.1 M KCl, [Anti-CD36] = 10 µg/mL, and [CD36] = 20 ng/mL).
Fig 2: (a) Effect of the CD36 concentration on the immunosensor current response, (b) linear detection range for CD36 by the PS/MWCNT-PAMAM/Anti-CD36-modified SPCE, (c) DPV profile corresponding to increasing concentrations of CD36 between 5 and 40 ng/mL, and (d) magnified image of the current peaks (all measurements, scan rate: 50 mVs–1, in the presence of 10 mL of PBS (pH 7.4) solution containing 5 mM HCF and 0.1 M KCl, [Anti-CD36] = 10 μg/mL; error bars show the SB of at least 3 replicate measurements).
Fig 3: (1) Schematic illustration of the production of PS/MWCNT-PAMAM/Anti-CD36-modified SPCE and detection of CD36. (2a) CV profile of bare SPCE (1), PS/MWCNT-PAMAM (2), PS/MWCNT-PAMAM/Anti-CD36 (3), and PS/MWCNT-PAMAM/Anti-CD36/CD36 (4); (2b) DPV profile of bare SPCE (1), PS/MWCNT-PAMAM (2), PS/MWCNT-PAMAM/Anti-CD36 (3), and PS/MWCNT-PAMAM/Anti-CD36/CD36 (4); (2c) Nyquist profile of bare SPCE (1), PS/MWCNT-PAMAM (2), PS/MWCNT-PAMAM/Anti-CD36 (3), and PS/MWCNT-PAMAM/Anti-CD36/CD36 (4); (2d) CV curve of PS/MWCNT-PAMAM/Anti-CD36-modified SPCE at different scan rates; and (2e) correlation between the square root of the scan rates versus the peak currents (all measurements were carried out in 10 mL of PBS (pH 7.4) containing 5.0 mM HCF and 0.1 M KCl, [CD36] = 20 ng/mL, and [Anti-CD36] = 10 μg/mL).
Fig 4: (a) Interference effect of BSA, GLC, urea, and INS on the detection of CD36 with the PS/MWCNT-PAMAM/Anti-CD36 immunosensor and (b) interference effect of BSA, GLC, urea, and INS alone on the PS/MWCNT-PAMAM/Anti-CD36 immunosensor response ([CD36]: 20 ng/mL, [BSA]: 4 g/dL, [GLC]: 100 mg/dL, [Urea]: 14 mg/dL, [INS]: 10 µU/mL, and [Anti-CD36]: 10 µg/mL) (all measurements, scan rate: 50 mVs–1, in the presence of 10 mL of PBS (pH 7.4) solution containing 5 mM HCF and 0.1 M KCl, [Anti-CD36] = 10 µg/mL; error bars show the SD of at least 3 replicate measurements).
Supplier Page from Abcam for Recombinant Human CD36 protein (His tag)